Foreword

This study was conducted for the Directorate of Military Programs, Head-quarters, U.S. Army Corps of Engineers (HQUSACE), under Project 4A161102AH68, "Processes in Pollution Abatement Technology"; Work Unit FKL, "Pathways and Controlling Factors in Biodegradation of Energetic Wastes."

The work was performed by the Industrial Operations Division (UL-I) of the Utilities and Industrial Operations Laboratory (UL), U.S. Army Construction Engineering Research Laboratories (CERL). The CERL principal investigator was Neal R. Adrian. Walter J. Mikucki is Chief, CECER-UL-I; John T. Bandy is Operations Chief, CECER-UL; and Gary W. Schanche is the responsible Technical Director, CECER-TD. The CERL technical editor was William J. Wolfe, Technical Resources.

Dr. Michael J. O'Connor is Director of CERL.

Contents

SF 298 1

Foreword 2

List of Figures and Tables 4

1 Introduction 5

Background 5
Objectives 6
Approach 7
Mode of Technology Transfer 7

2 Materials and Methods 8

Chemicals 8
Source of Inoculum 8
Serum Bottle Biodegradation Studies 8
Enrichment Culture Studies 9
Analytical Methods 9
Sampling 10

3 Results and Discussion 11

Serum Bottle Biodegradation Studies 11
Enrichment Culture Studies 12

4 Conclusions 18

References 19

Distribution