Figures
1 Molecular structure of the nitramine explosive RDX. 5
2 RDX biodegradation in serum bottles incubated under methanogenic conditions. Three transient appearing peaks were identified as the mono-, di, and trinitroso-RDX intermediates. The integrator units of the peak corresponding to the nitroso-intermediate is shown. 11
3 RDX biodegradation by the methanogenic enrichment culture when amended with acetate, ethanol, pryuvate, or glucose. The electron donors were added to a concentration of 1 mM. 13
4 Inhibition of methane formation by RDX in serum bottles amended with 10 mM ethanol. The RDX concentrations are also shown. 16
5 Methane formation by the enrichment culture in serum bottles amended with RDX and BESA containing 2 mM ethanol. 16
6 Biodegradation of RDX by the methanogenic enrichment culture in serum bottles amended with BESA and 2 mM ethanol. The RDX concentrations in the sterile and ethanol unamended controls are also shown. 17
Tables
1 Methane production in serum bottles containing wastewater from an explosives manufacturing wastewater treatment plant. The bottles were amended with RDX to approximately 80 _M. 12
2 Inhibition of methane production in the enrichment culture by RDX. The bottles were amended to a concentration of 1 mM with the respective electron donor and 25 _M RDX. The methane values reported were taken after 19 days incubation. 14
3 Methane recovery from 1 mM ethanol when added to the enrichment culture. 14
4 Methane formation from ethanol by the enrichment culture in bottles amended with RDX or BESA. Ethanol and RDX were added to a concentration of 2 mM and 25 _M, respectively. 17